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Zebrafish: A Premier Vertebrate Model for Biomedical

The zebrafish (Danio rerio) is a versatile model organism that has been used in biomedical research for several decades to study a wide range of biological phenomena. There are many technical advantages of using zebrafish over other vertebrate models. They are readily available, hardy, easy, and ine Conclusions: Collectively, these data validate the use of zebrafish as a vertebrate model to assess the effect of radiation alone or with radiation response modulators. Zebrafish embryos may thus provide a rapid, facile system to screen novel agents ultimately intended for human use in the context of therapeutic or accidental radiation exposure

Zebrafish (Danio rerio) as a Vertebrate Model Host To Study Colonization, Pathogenesis, and Transmission of Foodborne Escherichia coli O157 Foodborne infections with enterohemorrhagic <i>Escherichia coli</i> (EHEC) are a major cause of diarrheal illness in humans and can lead to severe complications such as hemolytic uremic syndrome Zebrafish (Danio rerio) as a Vertebrate Model Host To Study Colonization, Pathogenesis, and Transmission of Foodborne Escherichia coli O157 Authors: Daniel H. Stones, Alexander G. J. Fehr, Laurel Thompson, Jacqueline Rocha, Nicolas Perez-Soto, Vipin T. P. Madhavan, Kerstin Voelz, and Anne Marie Krachler Authors Info & Affiliation Recent work shows that a newly initiated genetic approach in zebrafish is already making significant contributions to understanding the development of the vertebrate heart, an organ that contains several vertebrate-specific features. These and other studies point to the utility of the zebrafish system for studying a wide range of vertebrate.

Zebrafish have become an important model for vertebrate hematology, filling the gap between in vitro and mammalian animal models. Recent studies have taken advantage of their convenient embryology, genetic flexibility and conserved biology to make many valuable contributions to hematopoietic research Zebrafish (Danio rerio) are an integrative vertebrate model ideal for toxicity studies.The zebrafish genome is sequenced with detailed characterization of all life stages. With their genetic similarity to humans, zebrafish models are established to study biological processes including development and disease mechanisms for translation to human health The zebrafish, as an excellent vertebrate model, is increasingly used for assessing chemical toxicity and safety Zebrafish Euthanasia: Rapid Chilling • Tropical fish species, like zebrafish can be euthanized by rapid chilling to between 2 - 4 C • Rapid chilling protocol - Place the fish in a small volume of water in a container of adequate size - Fish must not come into direct contact with ice - Add four times the volume of ice on top of wate

Novel use of zebrafish as a vertebrate model to screen

The zebrafish is an important and widely used vertebrate model organism in scientific research, for example in drug development, in particular pre-clinical development. It is also notable for its regenerative abilities, and has been modified by researchers to produce many transgenic strains Abstract. Zebrafish is developing as a major model for assessing toxicity of pharmaceuticals, drugs, and pollutants. Besides its applications in regulatory toxicity and drug discovery, its characteristics make it a unique system to analyze sublethal toxic effects that only can be studied applying holistic, in toto approaches

Zebrafish ( Danio rerio) as a Vertebrate Model Host To

Zebrafish (Danio rerio) is a valuable non-mammalian vertebrate model widely used to study development and disease, including more recently cancer.The evolutionary conservation of cancer-related programs between human and zebrafish is striking and allows extrapolation of research outcomes obtained in fish back to humans T he zebrafish is a dream system for scientists riding the next wave of genome-wide exploration. This photogenic creature is the first vertebrate that has proven tractable to the type of.. Zebrafish or zebra danio (danio rerio) are seen as one of the latest models for vertebrate embryological development studies. These embryos have the great advantage that they develop as see through embryos, that is, all internal development can be clearly observed from the outside in the living embryo Zebrafish (Danio rerio) has been a prominent model vertebrate in a variety of biological disciplines. Substantial information gathered from developmental and genetic research, together with near-completion of the zebrafish genome project, has placed zebrafish in an attractive position for use as a toxicological model

The zebrafish has been used as an efficient alternative vertebrate model for the study of human skeletal diseases, thanks to its easy genetic manipulation, high fecundity, external fertilization, transparency of rapidly developing embryos, and low maintenance cost Zebrafish as an Alternative Vertebrate Model for Investigating Developmental Toxicity—The Triadimefon Example. International Journal of Molecular Sciences. Maria Zoupa. Download PDF. Download Full PDF Package. This paper. A short summary of this paper. 37 Full PDFs related to this paper

Zebrafish (Danio rerio) are a small-bodied tropical, freshwater fish species originally from South Asia.The ease of care, year-round prolific breeding, and transparent, external development have made these fish a popular model vertebrate for many fields of biology The use of zebrafish and other animals - both invertebrate and vertebrate - (see Box 1) provides valuable insights into how basic cellular processes are regulated during development and how disrupting these processes can impact on embryogenesis The zebrafish is an ideal model organism to study the development of vertebrate-specific organs, such as the heart, because it is amenable to both loss-of-function and gain-of-function analyses Zebrafish have fast become one of the primary organisms of choice for modeling vertebrate functional genetics. Rapid ex-utero development, proficient egg laying, ease of genetic manipulation, and affordability make zebrafish an attractive vertebrate research tool. Furthermore, zebrafish share over 80 % of disease causing genes with humans Zebrafish (Danio rerio) are a small-bodied tropical, freshwater fish species originally from South Asia. The ease of care, year-round prolific breeding, and transparent, external development have made these fish a popular model vertebrate for many fields of biology

In the last 30 years, the zebrafish has become a widely used model organism for research on vertebrate development and disease. Through a powerful combination of genetics and experimental embryology, significant inroads have been made into the regulation of embryonic axis formation, organogenesis, and the development of neural networks. Research with this model has also expanded into other. Invertebrate chordates have one HOX cluster, but mammals have four, suggesting that cluster duplication facilitated the evolution of vertebrate body plans. This report shows that zebrafish have.. The zebrafish has unique advantages for understanding the evolution of vertebrate immunity and to model human diseases. In this review, we will firstly give an overview of the current knowledge on vertebrate innate immune receptors with special emphasis on the inflammasome and then summarize the main contribution of the zebrafish model to this field, including to the identification of novel. This serves to highlight one of the potential shortcomings of the zebrafish as a model for genetic analysis; clearly, in order to extrapolate the analysis of fish development to other vertebrates it will be necessary to identify mutations in all cognates of any given gene—and there is the obvious problem that in some cases, the phenotypes of.

dead end, a Novel Vertebrate Germ Plasm Component, Is

The zebrafish (Danio rerio) is a versatile model organism that has been used in biomedical research for several decades to study a wide range of biological phenomena.There are many technical advantages of using zebrafish over other vertebrate models. They are readily available, hardy, easy, and inexpensive to maintain in the laboratory, have a short life cycle, and have excellent fecundity The zebrafish is a powerful tool to screen compounds, especially for early development, since it is a vertebrate, has a high genetic homology with humans, and the assays can be automated to provide relatively high-throughput along with high-content analysis. An emerging model for toxicology and drug developmen Zebrafish, a powerful model animal in developmental biology, are being developed as models for human diseases, contributing to biomedical science. Inventive forward genetic screens in zebrafish are being used to unravel genetic and signaling pathways that control vertebrate development, disease and behavior (Patton and Zon, 2001) The zebrafish has emerged as an invaluable model to examine substance toxicity on vertebrate development, particularly on cardiac development. The zebrafish offers numerous advantages for toxicology research not found in other model systems

Figure 1 from Zebrafish as a model to study neuroblastoma

Zebrafish (Danio rerio) as a Vertebrate Model Host To

Zebrafish are poikilothermic vertebrates, as their body temperature is known to vary with ambient temperature . Similarly to mammalian adipocytes, differentiated zebrafish adipocytes express acrp30, cfd, and fbp11a marker genes, responsible for the production of adipokines such as adipsin and adiponectin [ 56 ] Zebrafish have become a popular alternative to higher animals in biomedical and pharmaceutical research. The development of stable mutant lines to model target specific aspects of many diseases, including diabetes, is well reported. However, these mutant lines are much more costly and challenging to maintain than wild-type zebrafish and are simply not an option for many research facilities Zebrafish (Danio rerio) is a kind of common aquarium fish originating from India and has become a prominent vertebrate model for studying diseases [4,5].Zebrafish is more phylogenetically distant from humans than rodent, but it has 82% orthologues of human disease-associated genes [].In addition, zebrafish has a short generation time of 2-4 months, is productive with a single mating pair. In just 24 hours, the zebrafish heart is beating and blood is circulating around the body. But as a vertebrate model, could they be as useful as mice

Zebrafish genetics and vertebrate heart formatio

establish zebrafish as a nonmammalian vertebrate model for studying bile salt metabolism and Fxr signaling. INTRODUCTION Bile salts are the end product of cholesterol catabolism in the liver of all vertebrates (1). Upon lipid ingestion, bile salts are released into the duodenum as emulsifiers to solubilize lipids and are then reab In vertebrates in general, D3 is highly expressed during embryonic/fetal development. In zebrafish embryos, material dio3 mRNA is present at 1 hpf at relatively high levels, that quickly drop towards 24 hpf. A low and stable dio3 mRNA expression in whole embryos has been recorded from 24 to 72 hpf (Walpita, 2008) Zebrafish (Danio rerio), which is an emerging alternative vertebrate model, has also been used to study the progressive deterioration of biological functions in aging 6, 7

The potential of zebrafish to be used as a vertebrate model for chemical screens was first assessed by Peterson and colleagues (Peterson et al., 2000). They used wild-type zebrafish embryos to screen for chemical modifiers of development using a strategy analogous to genetic screens, where mutations are identified that affect development of. considers aquatic species as live, vertebrate animals at hatching.2 Although this is an imprecise stage for zebrafish it can be approximated at 72 hours post fertilization. General Guidelines for Animal Study Proposals (ASPs): • For purposes of animal number accountability, all stages of development greater than thre Zebrafish larvae have evolved into a very attractive model for drug discovery. Recently published methods facilitate genetical engineering of zebrafish, allowing the study of targets of interest in an easily accessible vertebrate system: a multiorgan culture model

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Zebrafish as a model for vertebrate hematopoiesis

4. All other nonhuman vertebrates (including fish) at hatching or birth. Exception: Because of their delayed cognitive neural development, zebrafish embryos are not considered vertebrate animals until 7 days (168 hours) post- fertilization. Rules for ALL Vertebrate Animal Studies. 1. All vertebrate animal studies must have a research plan that. The zebrafish (Danio rerio) has become an increasingly accepted vertebrate model organism for biomedical research (1, 2). Despite being a member of the teleost class of fish species, there is great homology in development as well as cell- and organ-specific structural and physiological properties between zebrafish and humans Vertebrate Models - Aquatics. The mission of the Zebrafish International Resource Center (ZIRC) is to provide a central repository for wild-type, mutant, and transgenic strains of zebrafish (Danio rerio) and for materials and information about zebrafish research and husbandry. In addition, we provide veterinary diagnostic pathology services. The zebrafish genome encodes the largest repertoire of functional vertebrate aquaporins with dual paralogy to human isoforms. Our data reveal an early and specific diversification of these integral membrane proteins at the root of the crown-clade of Teleostei Thyroxine-immunofluorescence quantitative disruption test (TIQDT) was designed to provide a simple, rapid, alternative bioassay for assessing the potential of chemical pollutants and drugs to disrupt thyroid gland function. This study demonstrated that zebrafish eleutheroembryos provided a suitable vertebrate model, not only for screening the potential thyroid disrupting effect of molecules.

Zebrafish as an integrative vertebrate model to identify

  1. There is a body of literature highlighting the many similarities between fish and mammalian lipid metabolism, which serves to increase the utility of this powerful model vertebrate. Our data suggest that zebrafish yolk lipid transport and metabolism is a tractable model to study lipoprotein biology, regulation and metabolism
  2. Postlethwait,J. H. et al. Vertebrate genome evolution and the zebrafish gene map. Nature Genet. 18 , 345-349 (1998). CAS Article Google Schola
  3. Zebrafish are a relatively well-studied model organism, endowed with one of the highest quality finished vertebrate reference genomes . Zebrafish excel as a model to uncover gene function ( 59 , 60 ) and are now advancing research in many fields, including stem cell biology ( 61 , 62 ), cancer ( 63 , 64 ), infectious disease ( 65 , 66.
  4. But as a representative for fish—which comprise around half of all known vertebrate species—the zebrafish and its startling slumber hint that multistage sleep may have been present in a common.
  5. The vertebrate GAF binds to specific sequence motifs (Matharu et al., 2010) at many sites in the mouse and zebrafish HoxD clusters.We tested for in vivo GAF interaction with the potential binding sites in zebrafish embryos using ChIP assay. We identified four major clusters of binding sites for GAF in the HoxD intergenes in the zebrafish based on sequence motif search, three of which are also.
  6. The unusual structure of the Vertebrate SLC25A3 gene is discussed elsewhere in more detail . Most of the other SLC25A* Zebrafish, Chicken, Mouse or Human CISs are never transcribed, that is they are probably embedded in introns signaled by strong splicing signals

308 21.9% of the vertebrate homologs were highly expressed in the mouse limb of E14.5 embryo, 309 zebrafish embryo, and zebrafish intestine, respectively (S5 Fig), raising the possibility that 310 siphons retain a group of genes expressed during embryogenesis or have an ancestral function in 311 the intestinal system. Although 6 genes were. Zebrafish have been extensively used as a model system for research in vertebrate development and pathogen-host interactions. We describe the complete genome of a novel picornavirus identified during a viral metagenomics analysis of zebrafish gu

Most animals possess multiple opsins which sense light for visual and non-visual functions. Here, we show spectral characteristics of non-visual opsins, vertebrate Opn3s, which are widely distributed among vertebrates. We successfully expressed zebrafish Opn3 in mammalian cultured cells and measured its absorption spectrum spectroscopically. When incubated with 11-cis retinal, zebrafish Opn3. Numerous signaling pathways have been implicated in controlling early vertebrate embryogenesis. P.-F. Xu et al. (p. [87][1]) identify the minimal set of factors necessary to get uncommitted cells to organize a complete embryo. Two opposing gradients of the growth factors Nodal and Bone Morphogenetic Protein were sufficient to instruct zebrafish embryonic pluripotent cells to organize a.

Zebrafish ( Danio rerio ) as an excellent vertebrate model

  1. species to be live vertebrate animals at hatching. Although this is an imprecise stage in zebrafish, OLAW considers zebrafis h hatching to occur at 72 hours/3 days post fertilization (dpf). 1. Zebrafish 0-3 days post fertilization (dpf) are not considered live vertebrate animals an
  2. Among the vertebrate homologs of the Ciona TSGs in the neural complex, 48% and 63% showed high expression in the zebrafish and mouse brain, respectively, suggesting that the central nervous system is evolutionarily conserved in chordates. In contrast, vertebrate homologs of Ciona TSGs in the ovary, pharynx, and intestine were not consistently.
  3. Automating Manipulation of Larvae for Imaging Screens. Union Biometrica develops tools for model organism research. The new VAST BioImager platform (Vertebrate Automated Screening Technology) is designed for zebrafish researchers who need to image large numbers of 2-7 day old sedated zebrafish larvae. Fig. 1: Base VAST BioImager System
  4. Genetic studies of Hh signaling in zebrafish complement mutant analysis in the mouse and provide an approach to test the conservation and divergence of Gli function in vertebrates. Loss of zebrafish Hh signaling leads to ventral spinal cord defects, deficiencies in ventral forebrain specification, absence of an optic chiasm due to retinal axon.

Although the vertebrate skeleton arose in the sea 500 million years ago, our understanding of the molecular fingerprints of chondrocytes and osteoblasts may be biased because it is informed mainly by research on land animals. In fact, the molecular fingerprint of teleost osteoblasts differs in key ways from that of tetrapods, but we do not know the origin of these novel gene functions The Vertebrate Genome Annotation (VEGA) database was first made public in 2004 by the Wellcome Trust Sanger Institute. It was designed to view manual annotations of human, mouse and zebrafish genomic sequences, and it is the central cache for genome sequencing centers to deposit their annotation of human chromosomes

  1. ergic system is the orthopedia ( otp )-expressing diencephalic neuronal population that constitutes the dopa
  2. Zebrafish forward and reverse genetic studies have identified roles of many previously unknown genes in vertebrate cardiac development and function because, unlike mouse and chicken, zebrafish embryos can survive the first 7 days of their development without a functional cardiovascular system, receiving oxygen by passive diffusion through the.
  3. The zebrafish Danio rerio, a common aquatic pet, has evolved as a powerful tool for the discovery of genes regulating cellular suicide both during normal vertebrate development and after genetic or environmental insult. In this review, we describe the techniques that can be applied to studying cell death in zebrafish as well as highlighting.
  4. Abstract. Zebrafish, Danio rerio, a subtropical vertebrate has, during the last decades, emerged as an important model organism in neurobiological and biomedical research.The zebrafish neurotransmitter systems, including major small molecular substances and neuropeptides and their receptors, are very similar with those of mammals
  5. Developmental BiologyHighly touted as a model of vertebrate development, the zebrafish has actually worried a number of biologists for some time. Technical obstacles have prevented them from observing whether its tissues develop along typical vertebrate pathways; an odd growth pattern would ruin the fish's value as a model. But a recent series of embryological experiments has brought a rush of.

Zebrafish - Wikipedi

The zebrafish (Danio rerio) is the premier non-mammalian vertebrate model organism. This small aquatic fish is utilized in multiple disciplines in the Mayo community and by over a thousand laboratories around the world because of its biological similarity to humans, its advanced molecular genetics, the elucidation of its genome sequence, and the ever-expanding and outstanding new biological. The zebrafish (Danio rerio) genome includes five distinct ferlin members (dysferlin, otoferlin, myoferlin, Fer1l4, and Fer1l6). The genetic tractability of zebrafish and ease of phenotypic analysis has made zebrafish a valuable organism for the study of uncharacterized vertebrate genes Rapid lesioning of large numbers of identified vertebrate neurons: applications in zebrafish. Gahtan E (1), O'Malley DM. (1)Department of Biology, 414 Mugar Hall, Northeastern University, Boston, MA 02115, USA. Establishing a causal role between the activity of specific individual nerve cells and the behaviors they produce (or the neural. The zebrafish embryo is tractable to a range of experimental approaches, and in recent years, work in this system has provided significant new insights into vertebrate hindbrain development. Nevertheless, details remain to be uncovered for all of the major questions we have posed: How is the hindbrain territory initially specified

Zebrafish as a Vertebrate Model to Assess Sublethal

Zebrafish Models of Cancer—New Insights on Modeling Human

Figure 1

https://www.ibiology.org/development-and-stem-cells/vertebrate-organ-development-zebrafish-heart/Part 1: Vertebrate Organ Development: The Zebrafish Heart: Z.. The Zebrafish-H. William Detrich 1999 This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications Most animals possess multiple opsins which sense light for visual and non-visual functions. Here, we show spectral characteristics of non-visual opsins, vertebrate Opn3s, which are widely distributed among vertebrates. We successfully expressed zebrafish Opn3 in mammalian cultured cells and measured its absorption spectrum spectroscopically. When incubated with 11-cis retinal, zebrafish Opn3.

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Zebrafish--the Canonical Vertebrate Scienc

  1. e if a mutation in a specific gene can cause a patient's symptoms, we often need experimental animal models. While mice and rats have been common choices for modeling human diseases in the past, the use of zebrafish.
  2. Vertebrate sodium channel beta subunit genes were not identified in the genomes of invertebrate chordates and are unrelated to known subunits of the para sodium channel in Drosophila. The identification of conserved orthologs to all 4 voltage-gated sodium channel beta subunit genes in zebrafish and the lack of evidence for beta subunit genes in.
  3. Vertebrate Genetics and Genomics. We explore molecular phenotypes through the transcript sequencing of whole vertebrate embryos and tissues either by traditional RNA-seq or 3' end transcript counting. Taking advantage of the high quality reference genomes of the zebrafish and mouse, we are able to capture the full complement of polyadenylated.
  4. Zebrafish larvae (Danio rerio) are increasingly used to translate findings regarding drug efficacy and safety from in vitro-based assays to vertebrate species, including humans.However, the limited understanding of drug exposure in this species hampers its implementation in translational research. Using paracetamol as a paradigm compound, we present a novel method to characterize.
  5. In conclusion, the zebrafish maxillary barbel offers many of the advantages common to other vertebrate models of regeneration: speed, simplicity, transparency and easy access. Although the barbel has no human analog, the cell types it contains are critical for our development and maintenance, including skin, pigment cells, taste buds, nerves.

Zebrafish Development - Embryolog

  1. ed for RNases. We report (1) that all three zebrafish RNases are ribonucleolytically active, with one of them having an RNase activity comparable to that of bovine RNase A, the prototype of the superfamily, (2) that two zebrafish RNases have.
  2. To label different developmental states of muscle cells in zebrafish embryos, we generated a myf5-GFP;mylz2-mCherry double transgenic zebrafish line. At the 11-somite stage, myf5-GFP expression was restricted to the newly formed somite, and no mylz2-mCherry expression was detected (Figure 1A). Expression of mylz2-mCherry was first detected at 30 hr postfertilization (hpf) in the anterior.
  3. The 16th International Zebrafish ConferenceJune 16-22, 2021 | Online. Mark your calendar for the 16th International Zebrafish Conference (IZFC)! The meeting will be held online, June 16 - 22. You won't want to miss this essential gathering for all things zebrafish. Featuring two stellar keynote speakers, a wealth of plenary, spot at the IZFC
  4. Description. This animation shows how zebrafish can rapidly heal wounds to the heart. When the human heart is severely wounded — during, for example, a heart attack — it cannot regenerate the damaged muscle tissue. However, a damaged zebrafish heart can recover most of its original shape, size, and function over time
  5. ation of.
Analysis of zebrafish sidetracked mutants reveals a novel